Normally, a five-day incubation is definitely the duration of Lively development supplying the best focus of botulinal toxin. If enrichment society shows no expansion at 5 times, incubate yet another ten times to detect possible delayed germination of injured spores before discarding sample as sterile. For pure culture isolation conserve enrichment
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Botulinum toxin is supplied like a powder which is reconstituted at some time of treatment into a solution making use of sterile standard saline. Dilution volumes vary from one to four mL per a hundred-unit vial. The botulinum toxin dose injected into glabellar elaborate muscles to the treatment of frown lines relies on the specific botulinum toxin